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促进来源于少突胶质祖细胞(OP)的少突胶质细胞髓鞘再生,提高内源性修复是改善多发性硬化运动障碍的重要方法之一,其重要前提是明确人类中枢神经系统中有丰富的可分化为少突胶质细胞的OP细胞,而这需要有对中枢神经系统中的OP细胞进行鉴定的可靠方法。本研究采用多种方法对猫和人的视神经进行染色,根据相关结果获取可用于研究人尸体解剖标本OP细胞表达的抗原表型。OP细胞又被称为NG2细胞,广泛表达NG2蛋白,属于一个独立的胶质细胞亚型,由于其表达OP细胞系的转录因子Olig1和Olig2,因此与少突胶质细胞相关。虽然NG2细胞形态多样,与轴索及其它胶质细胞紧密联系,但所有NG2细胞都可以作为OP细胞进行分化,补充局部丢失的少突胶质细胞。研究发现,在人和猫的视神经及猫脊髓的白质和灰质中,NG2细胞在神经胶质细胞中所占比例不足5%,与少突胶质细胞的比例为1∶10。本研究结果显示,NG2细胞数量特别是相对少突胶质细胞不充足,这可对促进多发硬化内源性修复的方法提供线索。
To promote oligodendrocyte remodeling derived from oligodendrocyte progenitor cells (OPs) and to improve endogenous repair is one of the most important ways to improve the dyskinesia of multiple sclerosis. Its important premise is to clarify the central nervous system There are abundant OP cells that can differentiate into oligodendrocytes, and this requires a reliable method of identifying OP cells in the central nervous system. In this study, a variety of methods were used to stain the optic nerve of cats and humans, and the antigenic phenotypes that could be used to study OP cell expression in human autopsy specimens were obtained based on the results. Also known as NG2 cells, OP cells express NG2 protein extensively and belong to an independent subset of glial cells that are associated with oligodendrocytes due to their expression of the transcription factors Olig1 and Olig2 of the OP cell line. Although NG2 cells are diverse in shape and are closely associated with axons and other glial cells, all NG2 cells can differentiate as OP cells to supplement partially lost oligodendrocytes. The study found that NG2 cells account for less than 5% of glial cells and oligodendrocyte ratio of 1:10 in the white matter and gray matter of the optic nerve and cat’s spinal cord in both humans and cats. The results of this study show that the number of NG2 cells, especially the relative lack of oligodendrocytes, may provide clues to methods that promote multiple sclerosis endogenous repair.