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目的:探讨重组人CD40配体(rhCD40L)诱导U937细胞中基质金属蛋白酶(MMPs)表达的作用及其与诱导型环氧合酶-2(COX-2)途径的关系。方法:体外培养U937细胞,以不同浓度(0.1、0.2、0.4 mg/L)的rhCD40L和不同浓度(10-5 mol/L、10-4 mol/L、10-3 mol/L)的COX-2特异性抑制剂(NS-398)分别刺激24 h。在加入终浓度为0.4 mg/L rhCD40L的基础上,加入终浓度为10-4 mol/L的NS-398,共同刺激U937细胞24 h后,收集培养上清液,用酶谱法测定MMPs的活性。结果:rhCD40L可使MMP-2和MMP-9的活性明显增加(P<0.01),且呈剂量依赖性;NS-398可抑制MMP-2和MMP-9的活性,且抑制作用随剂量的增加而增强(P<0.05)。结论:rhCD40L以浓度依赖的方式诱导MMPs表达,rhCD40L对MMPs的诱导作用可能与COX-2途径有关。
Objective: To investigate the effect of recombinant human CD40 ligand (rhCD40L) on the expression of matrix metalloproteinases (MMPs) in U937 cells and its relationship with the inducible cyclooxygenase-2 (COX-2) pathway. Methods: U937 cells were cultured in vitro and cultured with different concentrations of rhCD40L (0.1,0.2,0.4 mg / L) and COX-2 of different concentrations (10-5 mol / L, 10-4 mol / L, 10-3 mol / 2 specific inhibitor (NS-398) were stimulated for 24 h. After adding 0.4 mg / L rhCD40L to the final concentration of 10-4 mol / L NS-398, U937 cells were stimulated with NS-398 for 24 h, and the culture supernatants were harvested. The levels of MMPs active. Results: rhCD40L increased the activity of MMP-2 and MMP-9 (P <0.01) in a dose-dependent manner. NS-398 inhibited the activity of MMP-2 and MMP-9, But increased (P <0.05). CONCLUSION: rhCD40L induces the expression of MMPs in a concentration-dependent manner. The inducing effect of rhCD40L on MMPs may be related to the COX-2 pathway.