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目的 研究肠组织及血浆中二胺氧化酶 (DAO)在肠缺血时的变化情况及其变化对肠活力的预测价值。方法 32只健康家兔随机分为 4组 ,各选取一段小肠制成完全缺血模型 ,提取各组缺血肠管组织以及肠系膜静脉血 ,液体闪烁计数法测定标本中DAO活性变化情况。结果 肠缺血时间愈长 ,缺血肠管肠组织及系膜血中DAO活性下降愈显著 (P <0 .0 5 ) ,再灌注 2 4h后 ,肠组织及血中DAO活性有不同程度恢复 ;DAO活性显著变化早于肠管出现的不可逆性改变 ;肠组织及血浆DAO活性变化呈正相关 ( r =0 .74,P <0 .0 5 )。结论 肠缺血可导致肠组织及血中DAO活性下降 ,DAO活性变化先于肠管出现的不可逆改变 ;DAO活性测定可作为预测迟发性肠坏死的有价值的指标。
Objective To study the changes of diamine oxidase (DAO) in intestinal tissue and plasma during intestinal ischemia and their predictive value on intestinal viability. Methods Thirty - two healthy rabbits were randomly divided into 4 groups. Each group of small intestine was selected to make a complete ischemia model. The ischemic intestinal tissue and mesenteric venous blood of each group were extracted. The changes of DAO activity in the samples were determined by liquid scintillation counting. Results The longer the duration of intestinal ischemia, the more obvious the decrease of DAO activity in the intestinal mucosa and blood in the intestinal mucosa (P <0.05). After 24 h of reperfusion, the activity of DAO in intestinal tissue and blood recovered to some extent. The change of DAO activity was earlier than the irreversible change of intestine. The change of DAO activity in intestinal tissue and plasma was positively correlated (r = 0.74, P <0.05). Conclusions Gut ischemia can lead to the decrease of DAO activity in the gut tissue and blood, and the change of DAO activity before the irreversible change of intestine. DAO activity can be used as a valuable index in predicting delayed intestinal necrosis.