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半夏为重要中药,系天南星科植物半夏的块茎,与其它中药配方,可用于镇吐。由于半夏种子或珠芽采收困难,迄今未能进行栽培。作者对半夏块茎切片的组织培养进行了研究。在组织培养的过程中,培养物上长出许多芽状的原球茎。三个月之中,原球茎组织上先长出了许多根,然后再发芽,培养获得成功。一、材料与方法在无菌条件之下,剪取半夏块茎的切片(直径约为1cm),去掉表皮层,加到含有0.5ppm 2,4-D和1ppm激动素的培养基中。将这些繁殖用的组织块的切片分别在液体培养基中培养4个星期。在整个培养过程中应保持其生长平衡。
Pinellia is an important traditional Chinese medicine, which is the tuber of Pinellia tuber, a plant of Araceae, and other traditional Chinese medicine formulas, which can be used for vomiting. Due to the difficulty in harvesting Pinellia seeds or bulbs, cultivation has so far been impossible. The authors studied the tissue culture of Pinellia tuber slices. In the course of tissue culture, many bud-shaped protocorms grow on the culture. During the three months, a number of roots were first grown on the original corm tissue and then germinated and the cultivation succeeded. I. Materials and Methods Under aseptic conditions, cut sections of Pinellia tubers (about 1 cm in diameter) were cut out, and the epidermis was removed and added to a medium containing 0.5 ppm of 2,4-D and 1 ppm of kinetin. The sections of these breeding tissue blocks were cultured in liquid medium for 4 weeks. The balance of growth should be maintained throughout the cultivation process.