论文部分内容阅读
建立了高效液相色谱法测定全血环孢霉素Ａ 浓度的方法。全血样品经多步处理后以２０ μＬ注入ｒｅｏｓｏｌｖｅ Ｃ１８，５μ色谱柱，采用Ｖ（乙腈）∶Ｖ（水）＝ ８０∶２０ 的流动相，在７５℃柱温下，以１ ｍ Ｌ／ｍ ｉｎ 流速洗脱，检测波长为２０８ｎｍ 。环孢霉素Ａ 的回收率为９９％ ～１０４ ％ ，相对标准偏差＜ ３．４２％ （ｎ＝ ５） ，日间＜ ８．１９％ （ｎ＝ ４） 。最低检测浓度为２５ μｇ／Ｌ。
A method for the determination of cyclosporin A concentration in whole blood by HPLC was established. The whole blood sample was subjected to multi-step processing and then injected into a reosolve C18, 5μ column at 20 μL using a mobile phase of V (acetonitrile): V (water) = 80:20 at a column temperature of 75 ° C at 1 m L / m in flow elution, detection wavelength of 208nm. The recoveries of cyclosporin A ranged from 99% to 104% with relative standard deviations <3.42% (n = 5) and daytime <8.19% (n = 4). The minimum test concentration is 25 μg / L.