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目的建立红茶中黄曲霉毒素B_1的测定方法。方法用60%乙腈-水提取茶叶中黄曲霉毒素B_1,经离心、稀释、过滤后通过免疫亲和柱,用甲醇将吸附在免疫亲和柱上的黄曲霉毒素B_1洗脱,洗脱液经光化学柱后衍生,通过带有荧光检测器的高效液相色谱仪定量分析。结果本方法检出限0.13μg/kg;线性范围0.5~20μg/L;相关系数r=0.999977;两种加标浓度平均回收率分别为85.0%和83.8%,相对标准偏差为分别为4.5%和3.2%。结论该方法消除了红茶中某些物质对免疫亲和柱吸附功能的干扰,具有简便、快捷、准确、灵敏度高和回收率好的特点,适用于红茶样品黄曲霉毒素B_1的检测。
Objective To establish a method for the determination of aflatoxin B1 in black tea. Methods Aflatoxin B1 in tea was extracted with 60% acetonitrile-water, centrifuged, diluted, filtered and then passed through an immunoaffinity column. The aflatoxin B1 adsorbed on the immunoaffinity column was eluted with methanol. Photochemical post-column derivatization was quantified by high performance liquid chromatography with a fluorescence detector. Results The detection limit of this method was 0.13μg / kg. The linear range was 0.5-20μg / L. The correlation coefficient was 0.999977. The average recoveries of the two spiked samples were 85.0% and 83.8%, respectively. The relative standard deviations were 4.5% and 3.2%. Conclusion The method can eliminate the interference of certain substances in the black tea on the adsorption of immunoaffinity column. The method is simple, rapid, accurate, sensitive and good in recovery. It is suitable for the determination of aflatoxin B1 in black tea samples.