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Liliaceous pollen cells were implanted by 4.0 MeV C 2+ ion beam or by 25.0 keV N + ion beam. Laser confocal scanning microscopy (LCSM) of the implanted intact samples showed that parts of the implanted pollen cells could be stained by propidium iodide (PI). This indicated that energetic ion beam could directly act on cells beneath the pollen coats and made channels for entry of the molecules from outside of the cells. LCSM analysis of green fluorescent protein (GFP) showed that energetic ion beam could mediate transient expression of gfp in treated pollen cells. Compared with 25.0 keV N + ion beam, implantation of 4.0 MeV C 2+ ion beam greatly improved gene transfer efficiency in pollen cells.
Liliaceous pollen cells were implanted by 4.0 MeV C 2+ ion beam or by 25.0 keV N + ion beam. Laser confocal scanning microscopy (LCSM) of the implanted intact samples showed that parts of the implanted pollen cells could be stained by propidium iodide (PI This indicates that energetic ion beam could directly act on cells beneath the pollen coats and made channels for entry of the molecules from outside of the cells. LCSM analysis of green fluorescent protein (GFP) showed that energetic ion beam could mediate transient expression of gfp in treated pollen cells. Compared with 25.0 keV N + ion beam, implantation of 4.0 MeV C 2 ion beam greatly improved gene transfer efficiency in pollen cells.