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作者用Ames法测定空气飘尘提取物的致突变性,多环芳烃(PAH)的分离用双相薄层层析法,用气谱-质谱定量分析。飘尘样品,在工业区(Dhmuta)一个采样点采集13份样品(2,220～4,750 m~3),在城市(Fukuoka)主要交通区的不同地点采集11份样品(1,120～2,820 m~3),每次收集24或48小时。这些样品用甲醇提取,用以对致突变性和苯并芘(B(a)P)含量相关性的研究。分离,在工业区的重污染区从5,646 m~3空气中采集粒子重6.422 g,用甲醇在索氏提取器内提取8小时,浓缩成提取物(1.434 g)
The authors used the Ames method to measure the mutagenicity of airborne dust extracts. The separation of polycyclic aromatic hydrocarbons (PAHs) was determined by gas chromatography-mass spectrometry using two-phase thin-layer chromatography. 13 samples (2,220 ~ 4,750 m ~ 3) were collected at one sampling point in the industrial area (Dhmuta) and 11 samples (1,120 ~ 2,820 m ~ 3) at different locations in the main traffic area of the city (Fukuoka) Collect for 24 or 48 hours. These samples were extracted with methanol for the study of the correlation between mutagenicity and benzopyrene (B (a) P) content. In the heavily polluted area of the industrial zone, 6.422 g particles were collected from 5,646 m ~ 3 air, extracted with methanol in a Soxhlet extractor for 8 hours, and concentrated into extract (1.434 g)