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目的探讨慢性阻塞性肺疾病(chronic obstructive plumonary disease,COPD)患者急性加重期治疗前后血清高迁移率族蛋白B1(high mobility group protein B1,HMGB1)的水平变化及其与肺功能的相关性。方法选取2014年10月—2015年6月COPD急性加重期患者72例,依据肺功能将其分为两亚组:GOLD 1、2级患者36例,GOLD 3级患者36例,另选取对照组36例,采用ELISA法分别测定所有对象的血清HMGB1水平,并行肺功能检测,计量资料比较采用t检验,相关关系采用Pearson相关性分析,P<0.05为差异有统计学意义。结果 COPD急性加重患者治疗前及治疗后血清HMGB1表达水平均明显高于对照组[(5.47±1.69)、(3.41±0.99)、(1.95±0.16)μg/L],对比差异均有统计学意义(均P<0.05)。GOLD 3级COPD患者治疗前及治疗后血清HMGB1表达水平明显高于GOLD 1、2级COPD患者治疗前及治疗后[(6.84±1.21)、(4.11±0.70)、(4.17±0.85)、(2.66±0.32)μg/L],对比差异均有统计学意义(均P<0.05)。COPD患者血清HMGB1水平与FEV1%pred呈显著负相关(r=-0.749,P<0.05)。结论血清HMGB1在COPD患者中表达水平明显升高,且与病情严重程度呈显著相关,提示检测血清HMGB1对COPD病情严重程度的判断有一定意义。
Objective To investigate the changes of serum high mobility group protein B1 (HMGB1) and its correlation with pulmonary function in patients with chronic obstructive pulmonary disease (COPD) before and after acute exacerbation. Methods Seventy-two patients with acute exacerbation of COPD from October 2014 to June 2015 were divided into two subgroups according to pulmonary function: 36 patients with GOLD stage 1 and 2, 36 patients with GOLD stage 3, and another control group Thirty-six healthy subjects were enrolled in this study. Serum levels of HMGB1 in all subjects were measured by ELISA, and pulmonary function tests were performed. The t test was used to compare the measurement data. Pearson correlation analysis was used to determine the correlation. P <0.05 was considered statistically significant. Results The serum levels of HMGB1 in patients with acute exacerbation of COPD before and after treatment were significantly higher than those in the control group [(5.47 ± 1.69), (3.41 ± 0.99), (1.95 ± 0.16) μg / L], respectively (All P <0.05). The levels of serum HMGB1 in patients with GOLD stage 3 COPD before and after treatment were significantly higher than those before and after treatment [(6.84 ± 1.21, 4.11 ± 0.70, 4.17 ± 0.85, 2.66 ± 0.32) μg / L], the differences were statistically significant (P <0.05). The level of serum HMGB1 in COPD patients was negatively correlated with FEV1% pred (r = -0.749, P <0.05). Conclusion Serum HMGB1 levels in COPD patients significantly increased, and with the severity of the disease was significantly correlated, suggesting that the detection of serum HMGB1 in COPD severity of the judgment of some significance.