Fingerprinting Analysis of Saposhnikovia divaricata using ~1H Nuclear Magnetic Resonance Spectroscop

来源 :Journal of Integrative Plant Biology | 被引量 : 0次 | 上传用户:zeldaok
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The 1H nuclear magnetic resonance(1H NMR) fingerprints of fractionated non-polar and polar extracts(control substance for plant drug [CSPD] A and B) from the roots of 12 specimens of Saposhnikovia divaricata(Turcz.) Schischk were achieved with Fourier Transform(FT)-NMR spectrometer and assigned by comparison to each other and to the 1H NMR spectra of the isolated individual compounds.These fingerprints were found to be uniform in terms of the specificity for the implication of all 12 specimens being systematically of the same origin.The uniformity was further affirmed by high performance liquid chromatography(HPLC),which also revealed exactly identical specificity for the identified S.divaricata species with the 1H NMR appearances of corresponding CSPD on the part of the composition of characteristic constituents when comparing to corresponding individual compounds.This investigation unambiguously shows that the specific signals from the chemotaxonomically significant compounds of chromones and coumarins in S.divaricata are exhibited distinctively in the composite features of both 1H NMR fingerprints and HPLC profiles.The 1H NMR and HPLC profiles established can successfully be used as reference for the authentication of the origin of S.divaricata species as well as for chemotaxonomic studies. The 1H nuclear magnetic resonance (1H NMR) fingerprints of fractionated non-polar and polar extracts (control substance for plant drug [CSPD] A and B) from the roots of 12 specimens of Saposhnikovia divaricata (Turcz.) Schischk were achieved with Fourier Transform (FT) -NMR spectrometer and assigned by comparison to each other and to the 1H NMR spectra of the isolated individual compounds. The fingerprints were found to be uniform in terms of the specificity for the implication of all 12 specimens being systematically of the same origin . The uniformity was further affirmed by high performance liquid chromatography (HPLC), which also showed exactly identical specificity for the identified S.divaricata species with the1H NMR appearances of the corresponding CSPD on the part of the composition of characteristic constituents when comparing to individual compounds. This investigation unambiguously shows that the specific signals from the chemotaxonomically significant compounds of chro mones and coumarins in S.divaricata are rendered distinctively in the composite features of both 1H NMR fingerprints and HPLC profiles. The 1H NMR and HPLC profiles established successfully successfully used as reference for the authentication of the origin of S.divaricata species as well as for chemotaxonomic studies.
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