当用Whatman DEAE——纤维(DE60)分离羊垂体蛋白时,发现全部他种垂体蛋白都被吸附。而生长素却全部存在于抽提液中。在4℃时,用硼砂缓冲液(离子强度0.025,pH8.6)抽提用干冰研磨过的垂体腺3小时。抽提液经透析和冰冻干燥。干燥物溶于硼砂缓冲液后再用DEAE——纤维处理。所得到的未经透析过的物质每毫克含0.6—0.8mg的生长素。其透析物的效价与NiHB_2生长素相 When the sheep pituitary protein was isolated by Whatman DEAE-fiber (DE60), all of his pituitary proteins were found to be adsorbed. And auxin is all present in the extract. At 4 ° C, the pituitary gland ground with dry ice was extracted with borax buffer (ionic strength 0.025, pH 8.6) for 3 hours. The extract is dialyzed and freeze-dried. The dried material is dissolved in borax buffer and then treated with DEAE-fiber. The resulting non-dialyzed material contains 0.6-0.8 mg auxin per mg. The dialysate titer and NiHB_2 auxin phase