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用乳过氧化物酶催化的~(125)I放射标记法,标记裂殖体及裂殖子为主的恶性疟原虫表面抗原。将标记原虫制成Triton x-100提取物,与流行区的11份病人血清和非流行区正常人血清进行免疫沉淀.通过SDS-PAGE和放射自显影分析恶性疟患者血清抗体的靶抗原.结果表明,上述恶性疟原虫的提取物中存在20多种表面抗原。分子量270、240、200、183、160、137、104、98、74和63KD的多肽系免疫沉淀中的主要靶抗原,其中137,104、98和63KD的多肽主要出现于三次感染以上的人群免疫血清中,表明上述多肽与诱导宿主产生的免疫力似有一定关系.
The lactoperoxidase-catalyzed ~ (125) I radiolabelling method was used to label the schizonts and merozoites of Plasmodium falciparum surface antigen. The tagged protozoa were made into Triton X-100 extracts and immunoprecipitated with 11 patient sera in endemic areas and normal human sera in non-endemic areas. The target antigens of serum antibodies to falciparum malaria patients were analyzed by SDS-PAGE and autoradiography. This shows that there are more than 20 kinds of surface antigens in the above mentioned Plasmodium falciparum extract. 160, 137, 104, 98, 74 and 63 KD of the peptide-based immunoprecipitates, of which 137, 104, 98 and 63 KD were mainly present in the immune serum of the population above the third infection , Indicating that the above-mentioned polypeptide and inducing host immunity seem to have a certain relationship.