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水稻叶片形态相关突变体的挖掘是进行水稻功能基因组学研究和株型改良的重要基础.本研究从60Co-γ辐射的籼稻粤丰B后代中鉴定一个卷叶突变体,命名为rl11(t),该突变体表型为株高降低、叶片卷曲变窄、叶脉数目减少且发育异常,同时对生长素的敏感性降低.遗传分析表明,该突变性状受一个隐性单基因控制.利用SSR标记将卷叶基因定位在位于水稻第4染色体上RM6089和RM124之间,在该基因附近区域发展了32对新的STS标记,将Rl11(t)精细定位在BAC克隆AL606645上STS4-25和STS4-26之间,物理距离约为31.6kb,为最终克隆目标基因奠定了基础.
Rice leaf morphology-related mutants mining is an important basis for rice functional genomics research and plant type improvement.In this study, a leaf-rolled mutant was identified from the indications of Yuefeng B60Co-γ radiation, named rl11 (t) , The phenotype of the mutant was decreased plant height, narrowed leaf curl, decreased number of veins and abnormal development, and reduced the sensitivity to auxin. Genetic analysis showed that the mutant was controlled by a recessive single gene. The leaf curl gene was mapped between RM6089 and RM124 located on chromosome 4 of rice. 32 pairs of new STS markers were developed in the vicinity of this gene and Rl11 (t) was finely mapped on BAC clone AL606645. STS4-25 and STS4- 26, the physical distance is about 31.6kb, which laid the foundation for the final cloning of the target gene.