论文部分内容阅读
为研究黄绿卷毛菇群体遗传结构,以青海、西藏、四川3个省份的10个地理群共91个样本黄绿卷毛菇为实验材料,采用分子生物学方法测定和分析所有样本的LSU和ITS序列。结果显示:1 995bp的LSU和ITS基因序列共有8个变异位点,得到20个基因型。单倍型多样性指数(Hd)和核苷酸多样性指数(pi)分别为0.538±0.026和0.00029±0.00002;青海玉树和青海兴海的遗传多样性指数最高。分子变异分析结果表明黄绿卷毛菇群体遗传变异主要来自种群内(75.70%),种群间遗传分化较大。系统进化树结果也表明,10个地理群分成2个支系,黄绿卷毛菇群体存在明显的地理结构。中性检测结果进一步证实,黄绿卷毛菇群体有着复杂的群体历史,曾发生过种群数量扩张,近期种群数量正在衰减。
In order to study the genetic structure of Pleurotus citrinopileatus population, 91 samples of Pleurotus citrinopileatus from 10 geographies in three provinces of Qinghai, Tibet and Sichuan were used as experimental materials. The molecular biological methods were used to determine and analyze the LSU And ITS sequences. The results showed that: 1 995 bp LSU and ITS gene sequences a total of 8 mutation sites, resulting in 20 genotypes. The haplotype diversity index (Hd) and nucleotide diversity index (pi) were 0.538 ± 0.026 and 0.00029 ± 0.00002, respectively. The genetic diversity index of Yushu in Qinghai and Xinghai in Qinghai was the highest. Molecular variance analysis showed that the genetic variation of Pleurotus citrinopileatus population mainly came from the inner part of the population (75.70%), and the genetic differentiation among the populations was large. The results of phylogenetic tree also showed that 10 geographies were divided into 2 sub-clades, and there was a clear geographical structure in the populations of Pleurotus ostreatus. Neutral test results further confirmed that the yellow green curl mushroom population has a complex group history, there have been population expansion, the recent population is declining.