目的:构建含粪肠球菌(Enterococcus faecalis,E.faecalis)ace基因的重组载体,在大肠杆菌M15中进行诱导表达,纯化表达产物并进行免疫活性分析,研究Ace蛋白在E.faecalis对胶原蛋白及HeLa细胞黏附过程中的作用,为探索Ace蛋白生物学功能及研发快速诊断试剂盒提供实验依据。方法:用Premier Primer 5.0引物设计软件分析E.faecalis的ace
By defining fuzzy valued simple functions and giving L1(μ) approximations of fuzzy valued integrably bounded functions by such simple functions, the paper ana
Because of the special underwater environment, many sensors used well in robots working in space or on the land can not be used in the underwater. So an optica