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目的：统计马王堆医院近年患者的病原菌分布和耐药性情况，分析其分布特点和耐药性的变化状况，为提高医院检验水平和了解细菌来源及抗菌药物的不同特点，探讨其变化趋势提供重要参考。方法：对本院近一年的患者细菌临床标本14021例的检测和药敏试验结果进行统计和分析。结果：共分离出阳性标本3420份。阳性率为24．39％。其中革兰阳性需氧菌436株，占比12．74％；革兰阴性需氧菌2418株，占比70．7％；真菌384株，占比11．23％；支原体183株，占比5．33％。而药物敏感性试验中，多重耐药性菌菌种分布比较广泛，一般主要集中在铜绿假单胞菌，鲍曼不动杆菌，大肠埃希菌，葡萄球菌属等。结论：主要细菌的耐药性总体呈下降趋势，加强对细菌分布及耐药菌的检测，了解其变化状况，对临床合理选用耐菌药物及控制医院感染具有非常重要的意义。“,”Objective:To investigate the distribution and antibiotic resistance of pathogenic bacteria from Mawangdui hospital patients in recent years in or-der to provide a scientific reference and promote the level of hospital inspection.Methods:To analysis the culture and the bacterial susceptibility of 14021 clini-cal specimens in the last year.Results:There were 3420 positive specimens in the 14021 cases(24.39%).Gram -positive aerobic bacteria were 436 (12. 74%).Gram-negative aerobic bacteria were 2418(70.7%).Fungi were 384(11.23%).Mycoplasma were 183(5.33%).There are various multi-drug re-sistant bacteria in Antimicrobial Susceptibility Testing,generally,main focus on Acinetobacter baumannii,Pseudomonas aeruginosa,Escherichia coli,Staphylococ-cus and so on.Conclusion:Bacterial resistance rate is rising.Drug resistance analyses and monitoring on pathogenic bacteria distribution is significant to guide clinical rational administration and to control the nosocomial infection.