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利用微机械加工技术,在ITO玻璃上设计制作了基于介质上电润湿效应的以离散液滴为对象的免疫检测芯片,对芯片的液滴驱动特性、免疫反应参数进行了测试,并对小鼠IgG和羊抗鼠IgG-HRP进行了初步的免疫测试。研究结果表明,在电压<100V的时候,接触角测量值基本上和预测曲线吻合,在>100V的情况下出现了接触角饱和现象,在芯片上实现了液滴操纵,120V时得到最大平均速度为3.75mm/s;该芯片可以实现免疫反应检测,所需样品体积为0.5μL,检测时间约为20min,对于羊抗鼠IgG-HRP实验系统的检测范围为0.1～20mg/L。
Using the micromachining technology, an immune detection chip based on the electrowetting effect of the medium on discrete droplets was designed and fabricated on the ITO glass. The droplet driving characteristics and immune response parameters of the chip were tested, Mouse IgG and goat anti-mouse IgG-HRP were subjected to a preliminary immunoassay. The results show that at voltages <100V, the contact angle measurements are basically in agreement with the predicted curves, with contact angle saturation occurring at> 100V, droplet manipulation on the chip and maximum average speed at 120V 3.75mm / s; The chip can be used to detect the immune response, the required sample volume is 0.5μL, the detection time is about 20min, the detection range of goat anti-mouse IgG-HRP experimental system is 0.1 ~ 20mg / L.