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Leaves of Acer truncatum‘Luhong No.1’contain large amounts of polysaccharides and polyphenols,which seriously affect extraction yield and quality of total RNA.In order to explore the appropriate total RNA extraction method,total RNA was extracted from leaves of A.truncatum‘Luhong No.1’with three methods,including kit method,Trizol method and modified CTAB method.The results showed that OD260/OD280 and OD260/OD230 ratios of total RNA extracted from leaves of A.truncatum‘Luhong No.1’with kit method were higher than 1.8,with a general yield and certain level of DNA contamination;OD260/OD280 and OD260/OD230 ratios of total RNA extracted with Trizol method were about 1.5,with the lowest yield;OD260/OD280 and OD260/OD230 ratios of total RNA extracted with modified CTAB method were about 2.0,with the highest yield and distinct electrophoresis patterns.The results demonstrated that total RNA extracted with modified CTAB method exhibited high yield and purity,which could meet the demands of subsequent molecular biology research.Thus,modified CTAB method is the appropriate method for extracting total RNA from leaves of A.truncatum‘Luhong No.1’.
Leaves of Acer truncatum’Luhong No. 1contain large amounts of polysaccharides and polyphenols, which seriously affect extraction yield and quality of total RNA. Order to explore the appropriate total RNA extraction method, total RNA was extracted from leaves of A.truncatum ’Luhong No.1’with three methods, including kit method, Trizol method and modified CTAB method. The results showed that OD260 / OD280 and OD260 / OD230 ratios of total RNA extracted from leaves of A.truncatum’Luhong No.1’with OD260 / OD280 and OD260 / OD230 ratios of total RNA extracted with Trizol method were about 1.5, with the top yield; OD260 / OD280 and OD260 / OD230 ratios of total RNA extracted with modified CTAB method were about 2.0, with the highest yield and distinct electrophoresis patterns.The results demonstrated that total RNA extracted with modified CTAB method exhibited high yield and purity, which could meet demands of subsequent molecular biology research.Thus, modified CTAB method is the appropriate method for extracting total RNA from leaves of A.truncatum’Luhong No.1 ’.