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目的建立高效液相色谱法同时测定薯片中罗丹明B、碱性橙2、碱性橙21、碱性橙22、酸性橙Ⅱ、苏丹红Ⅰ、苏丹红Ⅱ、苏丹红Ⅲ、苏丹红Ⅳ9种非法添加的工业染料。方法样品用丙酮-水(95∶5,V/V)混合溶液超声提取定容至10 ml,使用ZORBAX Eclipse Plus-C_(18)色谱柱(4.6 mm×250 mm,5μm),20 mmol/L乙酸铵水溶液(含0.1%甲酸)-甲醇为流动相,流速为1.0 ml/min,采用梯度洗脱,二极管阵列检测器测定,以保留时间定性,外标法峰面积定量。结果9种目标化合物在实验条件下能完全分离,在0.1μg/ml~5.0μg/ml内呈现良好的线性关系,相关系数均>0.999,检出限为0.05 mg/kg~0.25 mg/kg,3种浓度的加标回收率为80.2%~93.0%,相对标准偏差在2.8%~6.0%。结论本方法经实际应用证明,前处理过程简单,且准确度和精密度满足方法学要求,可应用于薯片中9种工业染料的实验室快速检测。
OBJECTIVE To establish simultaneous determination of rhodamine B, basic orange 2, basic orange 21, basic orange 22, acidic orange Ⅱ, Sudan Ⅰ, Sudan Ⅱ, Sudan Ⅲ, Sudan Ⅳ Ⅳ in potato chips by high performance liquid chromatography Illegal addition of industrial dyes. Methods The samples were extracted to a volume of 10 ml with a mixture of acetone and water (95: 5, V / V). The samples were separated on a ZORBAX Eclipse Plus-C 18 column (4.6 mm × 250 mm, Ammonium acetate aqueous solution (containing 0.1% formic acid) -methanol was used as the mobile phase at a flow rate of 1.0 ml / min. The gradient elution was performed with a diode array detector. The retention time was qualitative and the peak area was determined by external standard method. Results Nine target compounds were completely separated under the experimental conditions and showed a good linearity in the range of 0.1μg / ml ~ 5.0μg / ml with correlation coefficients> 0.999. The limits of detection were 0.05 mg / kg ~ 0.25 mg / kg, The spiked recoveries of three concentrations ranged from 80.2% to 93.0% with relative standard deviations from 2.8% to 6.0%. Conclusion The method proved by practical application, the pretreatment process is simple, and the accuracy and precision meet the methodological requirements, which can be applied to the laboratory rapid detection of nine kinds of industrial dyes in potato chips.