论文部分内容阅读
目的探讨运动性动情周期紊乱进程及恢复过程中雌性大鼠下丘脑-垂体-卵巢(HPO)轴各环节促性腺激素释放激素受体(GnRHR) mRNA水平变化的规律及意义。方法对不同训练周期及恢复过程的大鼠,采用RT-PCR检测其下丘脑、垂体、卵巢、子宫GnRHR mRNA的表达,采用液相平衡竞争放射免疫分析法检测其血清雌二醇(E2)、孕酮(P)水平。结果运动7周组大鼠下丘脑、垂体GnRHR mRNA表达及血清E2、P水平均显著降低(P<0.05~0.01);运动9周组大鼠下丘脑、垂体、卵巢GnRHR mRNA表达及血清E2、P水平均显著低于同期对照组(P<0.05~0.01);恢复2期组大鼠下丘脑、垂体GnRHR mRNA表达,血清E2及P水平仍低于同期对照组(P<0.05);恢复3期组大鼠E2、恢复4期组大鼠P水平及各运动及恢复组大鼠子宫GnRHR mRNA,与同期对照组相比均无显著性差异(P>0.05)。结果提示递增负荷运动大鼠HPO轴各环节GnRHR mRNA表达的变化与较低浓度的血清性腺类固醇水平,特别与E2水平密切相关,其表达水平的降低可能是运动性月经失调病理过程的重要一环。
Objective To investigate the regularity and significance of the changes of GnRHR mRNA in the hypothalamus-pituitary-ovarian (HPO) axis of female rats during the process of exercise estrous cycle disturbance and recovery. Methods The expression of GnRHR mRNA in the hypothalamus, pituitary, ovary and uterus was detected by RT-PCR in rats with different training cycles and recovery. The serum estradiol (E2) Progesterone (P) levels. Results The expression of GnRHR mRNA and the level of E2 and P in hypothalamus and pituitary were significantly decreased in the 7-week exercise group (P <0.05-0.01). The expression of GnRHR mRNA in hypothalamus, pituitary and ovary, (P <0.05 ~ 0.01). The mRNA expression of GnRHR in hypothalamus and pituitary of rats in the second phase was recovered, while the levels of E2 and P in serum were still lower than those in the control group (P <0.05) There was no significant difference in P level of rats in stage Ⅱ, group Ⅳ of recovery and GnRHR mRNA in uterus of recovery and exercise group compared with the control group (P> 0.05). The results suggest that the changes of GnRHR mRNA expression in HPO axis in increasing load rats are closely related to the lower concentration of serum gonadal steroids, especially E2, and the decreased expression may be an important part of the pathological process of exercise-induced menstrual disorders .