论文部分内容阅读
选用芸苔属细胞质类型不同的２个复合种甘蓝型油菜（Ｂ．ｎａｐｕｓＬ．Ｐ１）和埃塞俄比亚芥（Ｂ．ｃａｒｉｎａｔａＢｒａｕｎＰ２）正反交。Ｆ３代开始，从反交组合（Ｐ２×Ｐ１，ＣａＮ）选甘蓝型可育株对正交组合（Ｐ１×Ｐ２，ＮＣａ）的不育株测交。以后反交组合逐代选甘蓝型且具有保持能力单株作父本对正交组合不育株连续成对回交。ＮＣａ经过６代回交，获得稳定的雄性不育系。ＣａＮ经连续１１代选择成为稳定的保持系。细胞遗传学分析表明，不育系和保持系染色体数都为３８条且核型一致，保持系减数分裂染色体配对正常，表明ＮＣａ雄性不育系遗传稳定。ＮＣａ雄性不育系恢保关系测定表明，在甘蓝型油菜中有较多的恢复系且在恢保关系上与波里马雄性不育系有很大差异，在波里马保持系和恢复系中都有ＮＣａ雄性不育系的恢复系。
Two hybrid Brassica napus (B.napus L.) with different cytoplasm types of Brassica were crossed with B. bassarinata Braun P2. At the beginning of F3 generation, sterile plants of orthogonal combinations (P1 × P2, NCa) were selected from the male sterile lines of the reciprocal crosses (P2 × P1, CaN). After the anti-cross combinations were selected from the generation of the cabbage type and have the ability to maintain a single male parent for the orthogonal combination of sterile continuous pair backcross. After six generations of NCa backcrossing, a stable male sterile line was obtained. CaN has been selected as a stable maintainer by 11 consecutive generations. Cytogenetic analysis showed that the number of chromosomes in CMS lines and maintainer lines were 38 and their karyotypes were the same, keeping the paired meiosis chromosomes normal, indicating that the NCa male sterile lines were genetically stable. NCa male sterile line restore relationship determination showed that there are more in the Brassica napus restorer line and restore the relationship with the Polima male sterility are very different, in the Permian line and restorer line There are NCa male sterile line restorer lines.