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目的 :阐明α 2b干扰素 (IFNα 2b)抑制动脉粥样硬化 (AS)的体内机制。方法 :随机将 18只家兔分为空白对照 (NC)组、AS组和IFN治疗 (IFN)组 ,复制AS模型 ,于第 1、3、5、7周用酶法测血清总胆固醇、三酰甘油。 7周后主动脉苏丹Ⅳ染色测AS斑块面积 /总面积 ,苏木精 伊红染色观察主动脉形态学改变。用免疫组化法测胸主动脉中的增殖细胞核抗原 (PCNA)的表达 ,用原位杂交法测血小板生长因子 β(PDGF β)的表达程度。 结果 :主动脉粥样硬化斑块面积NC组和IFN组低于AS组 (P <0 .0 5 ) ,NC组与AS组差异无显著性意义。PDGF β的表达NC组和IFN组低于AS组 (P <0 .0 5 )。结论 :PDGF β可能是促使血管平滑肌细胞 (VSMC)增生、AS进展的主要原因。IFNα 2b通过下调PDGF βmRNA抑制VSMC的增生 ,可能是其抑制AS形成及发展的重要机制之一。
OBJECTIVE: To clarify the in vivo mechanism of interferon alfa 2b (IFNα 2b) inhibiting atherosclerosis (AS). Methods: Eighteen rabbits were randomly divided into blank control group (NC), AS group and IFN treatment group (IFN), AS models were replicated. Serum total cholesterol, triglyceride Acylglycerol. After 7 weeks, the area and area of AS plaques in the aorta were detected by Sudan Ⅳ staining. The morphology of the aorta was observed by hematoxylin and eosin staining. The expression of proliferating cell nuclear antigen (PCNA) in the thoracic aorta was detected by immunohistochemistry. The expression of platelet growth factor β (PDGF β) was detected by in situ hybridization. Results: The aortic atherosclerotic plaque area in NC group and IFN group was lower than that in AS group (P <0.05). There was no significant difference between NC group and AS group. The expression of PDGFβ in NC group and IFN group was lower than that in AS group (P <0.05). Conclusion: PDGF β may be the main reason for the proliferation of vascular smooth muscle cells (VSMCs) and the progression of AS. IFNα 2b may be one of the important mechanisms of its inhibition on the formation and development of AS by downregulation of PDGF β mRNA to inhibit the proliferation of VSMC.