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目的:探讨5-氨基乙酰丙酸(ALA)诱导卟啉代谢物[尿卟啉原(UP)Ⅰ和粪卟啉原(CP)Ⅲ]在大肠癌大鼠尿液中的积聚。方法:采用二甲基肼建立大鼠大肠癌模型。收集30只大肠癌大鼠(大肠癌组)和30只正常大鼠(正常组)的尿液。每只灌胃给药5-ALA(50 mg/kg)，2、4、6、8 h后留取尿液。用高效液相色谱法检测尿液中卟啉代谢产物UP Ⅰ和CP Ⅲ的含量，比较其含量在各组大鼠中的差异。结果:在口服5-ALA前，大肠癌组和正常组尿液中UP Ⅰ和CP Ⅲ的含量差异无统计学意义(n P>0.05)，口服5-ALA后大肠癌组尿液中UP Ⅰ和CP Ⅲ的含量较正常组明显升高(n P0.05). After oral administration of 5-ALA, the contents of UP Ⅰ and CP Ⅲ in urine of colorectal cancer group were significantly higher than those of normal group (n P<0.05). The contents of UP Ⅰ and CP Ⅲ in urine of colorectal cancer group reached the highest value at 4 hours. According to the receiver operating characteristic (ROC) curve drawn from 4-hour test results, the threshold value of UP Ⅰ for colorectal cancer diagnosis was 50.43 μmol/g, with corresponding sensitivity 96.7%, and the specificity 63.3%, respectively. The threshold value of CP Ⅲ for colorectal cancer diagnosis was 108.85 μmol/g, with corresponding sensitivity 66.7%, and the specificity 86.7%, respectively.n Conclusions:The accumulation of porphyrin metabolites induced by 5-ALA in the urine of rats with colorectal cancer is significant. The porphyrin metabolites in urine may be a new tumor marker of colorectal cancer, which provides an experimental basis for the diagnosis of colorectal cancer.