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大豆胞囊线虫(soybean cyst nematode,SCN)引起的病害能给大豆生产造成严重损失。探索影响大豆胞囊线虫(soybean cyst nematode,SCN)生理小种鉴定的因素对指导抗线育种工作具有重要意义。本研究以Lee68为材料,接种一定浓度的SCN4虫卵,在不同温度梯度的培养箱中培养,研究温度对大豆胞囊线虫生长的影响;以Riggs鉴定模式中的5个寄主为材料,接种一定浓度梯度的SCN2虫卵,研究接种浓度对鉴定结果的影响;从土壤相对含水量方面研究水分对大豆根系发育的影响。研究结果表明:在设定的温度梯度中,培养温度25℃/22℃(光/暗),Lee68上生长的胞囊数目最多,与其它几个温度培养条件相比,单株胞囊平均数达到差异显著水平,是胞囊生长最适宜温度;接种虫卵为1 200~24 000个/杯时,鉴定结果是正确的,超出这个接种范围,鉴定结果不稳定;一天浇水两次,浇水前/后土壤含水量为4%~7%/15%~18%,最有利于根系发育。本研究结果可为大豆胞囊线虫生理小种鉴定和抗线育种工作提供参考。
Diseases caused by soybean cyst nematode (SCN) can cause severe losses in soybean production. It is of great significance to explore the factors influencing the identification of physiological race of soybean cyst nematode (SCN). In this study, Lee68 as a material, inoculated with a certain concentration of SCN4 eggs, cultured in different temperature gradient incubator to study the temperature on the growth of soybean cyst nematode; Riggs identification model of the five host materials, vaccination certain Concentration gradient of SCN2 eggs to study the effect of inoculation concentration on the identification results; and to study the effect of water on root development of soybean from the aspects of soil relative water content. The results showed that the number of cysts grown on Lee68 was the highest at the temperature of 25 ℃ / 22 ℃ (light / dark) at the set temperature gradient. Compared with several other temperature culturing conditions, the average number of cysts per plant Reached a significant difference, is the most suitable temperature for cyst growth; when the inoculation of eggs is 1 200 ~ 24 000 / cup, the identification result is correct. Out of this inoculation range, the identification result is not stable; watering twice a day, Soil moisture before and after water is 4% ~ 7% / 15% ~ 18%, which is most beneficial to root development. The results of this study may provide a reference for the identification of race of soybean cyst nematode and breeding of resistant line.