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目的观察TEIF蛋白在软组织肉瘤组织中的表达及与类型、分级的关系。方法通过原核表达TEIF蛋白免疫制备多克隆抗体,免疫印迹鉴定。以组织芯片-免疫组织化学(EnVision法)检测166例软组织肉瘤和28例良性肿瘤及瘤样病变标本TEIF蛋白表达。结果制备多克隆抗体经Western印迹鉴定与TEIF具有特异结合性。免疫组织化学显示166例软组织肉瘤中TEIF蛋白阳性97例,阳性率为58%(97/166),高于良性肿瘤及瘤样病变(11%,3/28)(P<0.05)。阳性主要分布为滑膜肉瘤94%(16/17)、原始神经外胚叶瘤(PNET)91%(21/23),两者高于隆突性皮肤纤维肉瘤43%(6/14)、黏液性纤维肉瘤38%(6/16)、恶性外周神经鞘膜瘤36%(8/22)、脂肪肉瘤32%(6/19)(P<0.05),而与恶性纤维组织细胞瘤75%(15/20)、横纹肌肉瘤(7/10)、平滑肌肉瘤64%(9/14)差异无统计学意义(P>0.05)。同时,TEIF蛋白的弥漫阳性表达(≥2+)多存在于PNET和滑膜肉瘤,分别为83%(19/23)和76%(13/17)。在法国癌症中心联盟(FNCLCC)分级中,19例Ⅰ级肉瘤中TEIF阳性表达为32%(6/19),44例Ⅱ级肉瘤阳性率为48%(21/44),两者中2+以上阳性分别为11%(2/19)、32%(14/44),而70例Ⅲ级肉瘤中TEIF阳性表达为84%(59/70)和2+以上阳性为70%(49/70)。Ⅲ级组的TEIF阳性率(及2+以上阳性率)显著高于Ⅱ级组和Ⅰ级组(均为P<0.05),而后两者间则差异无统计学意义(P>0.05)。结论TEIF蛋白表达存在于软组织肉瘤,尤其高表达于PNET、滑膜肉瘤,并与肉瘤的组织学分级有关。
Objective To observe the expression of TEIF protein in soft tissue sarcoma and its relationship with type and grade. Methods The polyclonal antibodies were prepared by the prokaryotic expression of TEIF protein and identified by immunoblotting. Tissue chip - immunohistochemistry (EnVision method) detection of 166 cases of soft tissue sarcoma and 28 cases of benign tumor and tumor-like lesion TEIF protein expression. Results The polyclonal antibodies were identified by Western blot with specific binding to TEIF. Immunohistochemistry showed that the positive rate of TEIF protein in 166 soft tissue sarcomas was 97% (97/166%), which was higher than that of benign tumors and tumor-like lesions (11%, 3/28) (P <0.05). The main positive distribution was 94% (16/17) of synovial sarcoma and 91% (21/23) of primitive neuroectodermal tumor (PNET), both of which were higher than 43% (6/14) of protuberant dermatofibrosarcoma, Mucous fibrosarcoma 38% (6/16), malignant peripheral nerve sheath tumor 36% (8/22), liposarcoma 32% (6/19) (P <0.05), and with malignant fibrous histiocytoma 75% (15/20), rhabdomyosarcoma (7/10), and 64% (9/14) of leiomyosarcoma had no statistical significance (P> 0.05). At the same time, the diffuse positive expression of TEIF protein (≥2 +) was mostly found in PNET and synovial sarcoma, which were 83% (19/23) and 76% (13/17), respectively. In FNCLCC classification, the positive expression rate of TEIF in 19 cases of grade Ⅰ sarcoma was 32% (6/19), and the positive rate of 44 cases of grade Ⅱ sarcoma was 48% (21/44) The positive rates of TEIF were 11% (2/19) and 32% (14/44) respectively, while the positive rates of TEIF in 70 cases of grade Ⅲ sarcoma were 84% (59/70) and those of 2+ were higher than 70% (49/70 ). The TEIF positive rate (positive rate above 2+) in grade Ⅲ group was significantly higher than that in grade Ⅱ and Ⅰ group (both P <0.05), but there was no significant difference between the latter two groups (P> 0.05). Conclusion The expression of TEIF protein exists in soft tissue sarcoma, especially in PNET and synovial sarcoma, which is related to the histological grade of sarcoma.