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为了评价人面果叶子、根部、果实提取物体外抗糖尿病活性,相应测定了其石油醚提取物(PFr.)、乙酸乙酯提取物(EFr.)、正丁醇提取物(BFr.)、水提取物(WFr.)的α-葡萄糖苷酶与α-淀粉酶抑制活性,以及HepG2细胞的促葡萄糖消耗能力。果实乙酸乙酯提取物(IC50=17.81±1.09μg/mL)、叶子乙酸乙酯提取物(IC50=18.60±1.56μg/mL)、根部乙酸乙酯提取物(IC50=14.05±0.24μg/mL)、根部正丁醇提取物(IC50=13.01±0.38μg/mL)显示了较好的α-葡萄糖苷酶抑制活性(acarbose IC50>200μg/mL)。而根部乙酸乙酯与正丁醇提取物在600μg/mL的浓度下就显示了90%的α-葡萄糖苷酶抑制率,在1.5 mg/mL的浓度下显示了90%的α-淀粉酶抑制率。在促葡萄糖消耗试验中,果实乙酸乙酯提取物在浓度为7.5~30 mg/mL时显示了很好的促HepG2细胞葡萄糖消耗能力(P<0.001),叶子乙酸乙酯提取物、根部正丁醇与乙酸乙酯提取物的促葡萄糖消耗率达到了3.08、3.12、1.93,仅次于果实乙酸乙酯提取物(3.91)。此次研究为人面果抗糖尿病活性开发提供一定理论基础。
In order to evaluate the anti-diabetic activity of the leaf, root and fruit extracts of noodles, the petroleum ether extract (PFr.), Ethyl acetate extract (EFr.), N-butanol extract Α-glucosidase and α-amylase inhibitory activity of water extract (WFr.), And glucose-promoting ability of HepG2 cells. Ethyl acetate extract (IC50 = 17.81 ± 1.09 μg / mL), ethyl acetate extract (IC50 = 18.60 ± 1.56 μg / mL) and ethyl acetate extract (IC50 = 14.05 ± 0.24 μg / mL) , N-butanol extracts (IC50 = 13.01 ± 0.38 μg / mL) showed better α-glucosidase inhibitory activity (acarbose IC50> 200 μg / mL). While the root ethyl acetate and n-butanol extracts showed 90% alpha-glucosidase inhibition at a concentration of 600 μg / mL and 90% alpha-amylase inhibition at a concentration of 1.5 mg / mL rate. In the glucose-boosting test, the ethyl acetate extract of fruits showed good glucose-depleting ability to HepG2 cells (P <0.001) at concentrations of 7.5-30 mg / mL, ethyl acetate extract of leaves, Alcohol and ethyl acetate extract to promote glucose consumption rate of 3.08,3.12,1.93, second only to fruit ethyl acetate extract (3.91). This study provides a theoretical basis for the development of human anti-diabetic activity of noodles.