论文部分内容阅读
背景党参和黄芪是临床常用的补益中药,均有改善心肌缺血的作用。目的比较党参和黄芪提取物对实验性心肌缺血大鼠的保护作用。设计完全随机分组设计,对照实验。单位哈尔滨商业大学药学院。材料实验于2002-09/2002-12在哈尔滨商业大学药学院药理学实验室完成。选用健康成年W istar大鼠30只。随机将大鼠分为3组对照组、党参组、黄芪组,每组10只。方法①麻醉大鼠后,记录Ⅱ导联心电图,对照组、党参组、黄芪组分别腹腔注射生理盐水30g/kg,党参提取物30g/kg,黄芪提取物30g/kg[党参、黄芪水提取物(原生药含量1g/m L)],待1～3m in,立即腹腔注射垂体后叶素0.5U/kg(建立心肌缺血模型),用生理盐水稀释至0.1～0.2m L,10s注完,采用LM S-2B型二道生理记录仪记录给垂体后叶素后心电图变化(T波和ST段的位移和心率的变化)。②计量资料差异比较采用t检验。主要观察指标各组大鼠给药前后心电图T波和ST段的位移和心率的变化。结果大鼠30只均进入结果分析。①T波高度3组给药后明显高于给药前(P<0.01),黄芪组明显小于党参组[(1.63±3.81),(2.18±2.93)m m,P<0.01]。②心率3组给药后明显低于给药前(P<0.01),黄芪组明显低党参组[(212.21±5.01),(221.18±5.66)次/m in,P<0.01]。③ST段高度3组间差异不明显(P>0.05),给药后与给药前差异也不明显(P>0.05)。结论①黄芪和党参均可对垂体后叶素所致的实验性心肌缺血起到明显保护作用。②党参对于减小T波抬高的作用强于黄芪,而对于减慢心率的作用要弱于黄芪。
BACKGROUND Codonopsis pilosula and Astragalus membranaceus are commonly used traditional Chinese herbal medicines for tonifying and improving myocardial ischemia. Objective To compare the protective effects of extracts of Radix Codonopsis and Radix Astragali on experimental myocardial ischemia in rats. Design completely random grouping design, control experiments. Unit School of Pharmacy, Harbin University of Commerce. Material experiments were performed at the Pharmacology Laboratory, School of Pharmaceutical Sciences, Harbin University of Commerce from September 2002 to December 2002. 30 healthy adult Wistar rats were selected. Rats were randomly divided into 3 groups: control group, Dangshen group and Huangqi group, with 10 rats in each group. Method 1 After anesthetizing rats, II lead electrocardiogram was recorded. In the control group, Dangshen group and Huangqi group, normal saline 30g/kg, Dangshen extract 30g/kg, Radix Astragali extract 30g/kg [Dangshen and Radix Astragali water extracts were intraperitoneally injected respectively. (1g/m L of original drug content)], wait 1~3m in, immediately intraperitoneal injection of pituitrin 0.5U/kg (build myocardial ischemia model), dilute to 0.1 ~ 0.2m L with normal saline, 10s finished The changes of electrocardiogram (changes in T-wave and ST-segment displacements and heart rate) were recorded after administration of pituitrin with a LM S-2B 2-channel physiological recorder. 2 Measurement data differences were compared using t test. Main observation indexes Electrocardiographic T wave and ST segment displacement and heart rate before and after administration of rats. Results Thirty rats were involved in the result analysis. 1T wave height in three groups was significantly higher than before administration (P<0.01), and Huangqi group was significantly smaller than Dangshen group [(1.63±3.81), (2.18±2.93) mm, P<0.01]. 2Heart rate was significantly lower in the 3 groups after administration than before the administration (P<0.01). The Huangqi group was significantly lower in the Dangshen group [(212.21±5.01), (221.18±5.66) beats/m in, P<0.01]. 3ST segment height was not significantly different between the 3 groups (P>0.05), and there was no significant difference between the administration and the administration (P>0.05). Conclusion 1 Both Astragalus and Codonopsis can protect the experimental myocardial ischemia induced by pituitrin. 2 The role of Dangshen in reducing T-wave elevation is stronger than that of Huang Li, but it is less effective in slowing down the heart rate than Huang Li.