论文部分内容阅读
采用一次性大鼠尾静脉注射内毒素（Ｌｉｐｏｐｏｌｙｓａｃｃｈａｒｉｄｅ，ＬＰＳ）２５０μｇ／ｋｇ体重，导致肾功能障碍，用放免法测定肾组织中ＴＸＢ２、６－ｋｅｔｏ－ＰＧＦ１α的含量。结果发现：注射ＬＰＳ后７２ｈ内肾组织中ＴＸＢ２、６－ｋｅｔｏ－ＰＧＦ１α含量及两者比值均显著增加，且以１ｈ最明显，同时见注射ＬＰＳ后１ｈ肾小球毛细血管非常显著收缩，提示肾组织中ＴＸＢ２、６－ｋｅｔｏ－ＰＧＦ１α含量增加及两者比例失衡是肾小球毛细血管收缩的重要原因之一。先注入氮芥减少白细胞后再注入ＬＰＳ，则肾小球内中性粒细胞和血小板明显低于ＬＰＳ，此时肾组织中ＴＸＢ２、６－ｋｅｔｏ－ＰＧＦ１α含量改变不明显，提示肾小球内中性粒细胞和血小板可能是肾组织中ＴＸＢ２、６－ｋｅｔｏ－ＰＧＦ１α的主要来源。
One-time injection of 250μg / kg body weight lipopolysaccharide (LPS) into the caudal vein of rats resulted in renal dysfunction. The content of TXB2, 6-keto-PGF1α in renal tissue was determined by radioimmunoassay. The results showed that the content of TXB2, 6-keto-PGF1α and the ratio of them both increased significantly within 72 hours after LPS injection, and most obviously at 1 hour. At the same time, the glomerular capillaries contracted remarkably 1 h after injection of LPS, Tissue TXB2, 6-keto-PGF1α levels and the imbalance between the two is one of the important reasons for glomerular capillary contraction. The first injection of nitrogen mustard to reduce leukocyte injection of LPS, glomerular neutrophils and platelets was significantly lower than LPS, then the content of TXB2, 6-keto-PGF1α in renal tissue was not significantly changed, suggesting that glomerular Granulocytes and platelets may be the main source of TXB2,6-keto-PGF1α in renal tissues.