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目的探讨磷酸化信号传导和转录激活因子3(p-stat3)和E钙黏蛋白在表皮肿瘤的表达和意义。方法免疫组化方法观察30例皮肤鳞状细胞癌(SCC)、20例基底细胞上皮瘤(BCC)、20例脂溢性角化病(SK)肿瘤细胞中p鄄stat3和E钙黏蛋白的表达,20例正常人皮肤为对照。结果①p鄄stat3在皮肤SCC、BCC中呈明显的上调表达,皮肤SCC中p鄄stat3的表达强度明显高于BCC(P<0.05);②p鄄stat3在皮肤SCC中的表达强度与肿瘤的分化程度有关(P<0.05),阳性表达率与肿瘤浸润的深度有关(P<0.05),与肿瘤的大小无关。③皮肤SCC中E钙黏蛋白的表达明显减弱(P<0.001),且皮肤SCC中E钙黏蛋白的表达强度比皮肤BCC也明显减弱(P<0.05)。在皮肤SCC中E钙黏蛋白的表达强度与肿瘤的分化程度有关,阳性表达率与肿瘤浸润的深度和肿瘤的大小无关。④皮肤SCC中,p鄄stat3和E钙黏蛋白的阳性表达强度具有负相关性,rs=-0.37,P<0.05。结论p鄄stat3的表达异常可能在表皮肿瘤的发生中起重要作用。stat3的过度活化可能与皮肤SCC的侵袭性潜能密切相关。
Objective To investigate the expression and significance of phosphorylation signal transducers and activators of transcription 3 (p-stat3) and E-cadherin in epidermal tumors. Methods Immunohistochemistry was used to detect the expression of p stat3 and E cadherin in 30 cases of squamous cell carcinoma (SCC), 20 cases of basal cell carcinoma (BCC) and 20 cases of seborrheic keratosis (SK) Expression, 20 normal skin as a control. Results ①p-stat3 was significantly upregulated in SCC and BCC, the expression level of p-stat3 in SCC was significantly higher than that in BCC (P <0.05); ② The expression of p-stat3 in SCC and the differentiation of tumor (P <0.05). The positive rate was related to the depth of tumor infiltration (P <0.05), but not to the size of tumor. ③ The expression of E-cadherin in skin SCC was significantly decreased (P <0.001), and the expression of E-cadherin in skin SCC was also significantly weaker than that of skin BCC (P <0.05). The expression of E-cadherin in skin SCC was related to the degree of tumor differentiation. The positive expression rate was not related to the depth of tumor invasion and tumor size. ④ In skin SCC, the positive expression of p-stat3 and E-cadherin had a negative correlation, rs = -0.37, P <0.05. Conclusion The abnormal expression of p-stat3 may play an important role in the development of epidermal tumors. Over-activation of stat3 may be closely related to invasive potential of cutaneous SCC.