以高活力玉米自交系J002和低活力玉米自交系196为试验材料,采用TMT标记定量蛋白质组学技术,对不同活力玉米种子萌发期进行淹水胁迫72 h处理,比较蛋白质表达的差异。结果表明,共鉴定到203个差异蛋白,其中,上调蛋白169条,下调蛋白34条。通过对差异表达蛋白的功能富集分析和KEGG代谢通路富集分析发现,高活力种子在萌发过程中核糖体蛋白的合成量要高于低活力种子。在缺氧胁迫下,高活力种子的ROS清除剂如POD、SOD、APX等丰度均高于低活力种子。KEGG通路分析表明,高活力种子激活了糖酵解代谢、丙酮酸代谢等更多的代谢途径来获得能量。鉴定出一些丰度具有巨大差异的热激蛋白HSPs,由此可以推断,高活力种子具有更好抗性的分子机制。 High-energy maize inbred line J002 and low-activity maize inbred line 196 were used as experimental materials. The TMT-labeled quantitative proteomics technique was used to investigate the differences in protein expression between different vigor maize seeds under waterlogging for 72 h. The results showed that a total of 203 differential proteins were identified, of which 169 proteins were up-regulated and 34 proteins were down-regulated. Through the enrichment analysis of differentially expressed proteins and the enrichment analysis of KEGG metabolic pathway, it was found that the synthesis amount of ribosomal protein in high vigor seeds was higher than that in low vigor seeds during germination. Under hypoxia stress, ROS scavengers such as POD, SOD and APX of high-vigor seeds were higher than low-vigor seeds. KEGG pathway analysis shows that high-vigor seeds activate more metabolic pathways such as glycolysis, pyruvate metabolism and more to gain energy. Some heat shock protein HSPs with large differences in abundance were identified, from which one can conclude that the molecular mechanism of higher vigor seeds is better.