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FSH是促进人和灵长类动物精子成熟的主要因素,鉴于注射抗FSH血清可以在短期内抑制精子的生成并干扰莱第(Leydig)细胞的内分泌功能,作者研究了在两年时间内用FSH主动免疫对成年雄性恒河猴睾丸功能的影响。将高度纯化的羊FSH250μg加入福氏完全佐剂给4只动物进行皮内多点注射作基础免疫,用不完全佐剂加强免疫,每次间隔1～2月,共计7次。以~(125)I-hFSH鉴定抗体滴度;用Steelman-Pohley生物测定法确定抗FSH抗体在体内中和FSH的能力。另选4只动物作为对照。以RIA法测定血中睾酮含量;以刺激小
FSH is a major factor in promoting sperm maturation in humans and primates. Given that anti-FSH serum can suppress sperm production in a short term and interfere with the endocrine function of Leydig cells, the authors studied the effects of FSH Effect of Active Immunization on Testis Function in Adult Male. Highly purified goat FSH250μg was added to Freund’s complete adjuvant to four animals for multiple intradermal injection for basic immunity, with incomplete adjuvant to enhance immunity, every 1 ~ 2 months, a total of 7 times. Antibody titers were identified by ~ (125) I-hFSH; the ability of anti-FSH antibodies to neutralize FSH in vivo was determined using the Steelman-Pohley bioassay. Another 4 animals as a control. Determination of blood testosterone by RIA method to stimulate small