论文部分内容阅读
目的:探讨大鼠胃窦黏膜AMP-18的减少或缺失与胃癌分化程度的关系。方法:将SD大鼠分成A、B、C、D 4组(每组5只),体重160~250 g,用N-甲基-N′-硝基-N-亚硝基胍(MNNG)诱导大鼠胃癌模型。将MNNG用蒸馏水配成100μg/m L的溶液,内含4 mg/L的吐温20及20 mg/L的维生素D3,装入避光饮水瓶中喂饲大鼠8周;第9~16周将MNNG的浓度增至150μg/m L;第17~24周给予180μg/m L的MNNG溶液。14、18、22和24周分别处死4组大鼠进行取材。各组分别进行HE染色切片和Q-PCR法检测大鼠胃窦黏膜AMP-18 m RNA的表达情况。结果:通过参照胃癌分期标准,HE染色切片显示,A、B组胃窦黏膜细胞无明显变化,C组为早期胃癌,D组为进展期胃癌,Q-PCR也显示C组、D组胃黏膜的AMP-18 mRNA表达均明显下降。结论:AMP-18 mRNA在早期和进展期胃癌表达明显下降。
Objective: To investigate the relationship between the decrease and loss of AMP-18 in antral gastric mucosa and the differentiation of gastric cancer. Methods: SD rats were divided into groups A, B, C and D 4 (5 rats in each group) weighing 160-250 g. The rats were anesthetized with N-methyl-N’-nitro-N-nitrosoguanidine Induced rat gastric cancer model. The MNNG was dubbed 100μg / mL with distilled water containing 4 mg / L of Tween 20 and 20 mg / L of vitamin D3, and the rats were housed in a light-proof drinking bottle for 8 weeks. MNNG concentration was increased to 150μg / m L week; 180μg / m L MNNG solution was administered from week 17 to 24; 14, 18, 22 and 24 weeks were sacrificed 4 rats were drawn. The expression of AMP-18 mRNA in antral mucosa of rats was detected by HE staining and Q-PCR respectively. Results: According to the staging criteria of gastric cancer, HE staining showed that there was no significant change in gastric mucosa cells in groups A and B, early gastric cancer in group C, advanced gastric cancer in group D, and gastric mucosa in groups C and D Of AMP-18 mRNA expression were significantly decreased. Conclusion: The expression of AMP-18 mRNA in early stage and advanced stage of gastric cancer is obviously decreased.