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目的 :探讨二期梅毒皮疹的形成机制和细胞免疫在梅毒皮疹形成中的作用。方法 :用巢式PCR方法对2 4例 10 %甲醛溶液固定、石蜡包埋的二期梅毒疹标本进行了梅毒螺旋体DNA检测 ;用免疫组化方法对二期梅毒皮损中的浸润细胞进行检测。结果 :2 4份二期梅毒疹标本中 10例 (4 5 .8% )检出了梅毒螺旋体DNA ,所有标本银染色未发现苍白螺旋体 (TP)。二期梅毒皮损浸润细胞中CD4 5RO(+)T细胞 10 0 %阳性 ,6 8.2 %有CD6 8(+)巨噬细胞 ,此外还有少量CD2 0 (+)B淋巴细胞和CD5 7(+)NK细胞。结论 :二期梅毒疹的形成原因可能为螺旋体感染皮肤局部所致 ;二期梅毒疹皮损中浸润细胞主要为T淋巴细胞和巨噬细胞 ,细胞免疫在二期梅毒皮损发病中可能发挥重要作用
Objective: To investigate the mechanism of secondary syphilis rash and the role of cellular immunity in the formation of syphilis rash. METHODS: Twenty-four cases of syphilis with paraffin-embedded secondary syphilis were fixed in 24 cases of 10% formaldehyde solution by nested PCR. The infiltrating cells in the second stage of syphilis lesions were detected by immunohistochemistry . Results: Treponema pallidum DNA was detected in 10 samples (24.5%) of 24 samples of secondary syphilis, and all samples were found no silver spirochetes (TP). There were 100% positive CD4 5RO (+) T cells in infiltrating cells of secondary syphilitic lesion and 6 8.2% of CD6 8 (+) macrophages in addition to a small amount of CD20 (+) B lymphocytes and CD5 7 (+ ) NK cells. Conclusion: The formation of secondary syphilis may be caused by local infection of spirochete skin. The infiltrating cells in secondary syphilis rash are mainly T lymphocytes and macrophages. Cellular immunity may play an important role in the pathogenesis of secondary syphilis lesions effect