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部分纯化的蛋白激酶A和蛋白激酶C，在体外分别与瑞香素温育。瑞香素浓度达0．56×10-8mol／L时，显著抑制Ⅱ型蛋白激酶A的活力，大于0．56×10－5mol／L时，抑制Ⅰ型蛋白激酶A的活力，而高至0．56×10-4mol／L时，才能抑制蛋白激酶C的活力。结果表明，瑞香素对Ⅱ型蛋白激酶A有较高的特异抑制作用。
Partially purified protein kinase A and protein kinase C were incubated with tribenurin in vitro. Daphnetin concentration of 0.56×10-8 mol/L significantly inhibited the activity of type II protein kinase A. When the daphnetin concentration was greater than 0.56×10-5 mol/L, the activity of type I protein kinase A was inhibited, while it was as high as 0 At 56×10-4 mol/L, protein kinase C activity can be inhibited. The results showed that Daphnetin had a high specific inhibitory effect on type II protein kinase A.