两性霉素B是重要的抗霉菌抗生素,为了减少其毒性,现采用与磷脂(二肉豆蔻酰磷脂酰胆碱DMPC和二肉豆蔻酰磷脂酰甘油DMPG)结合成脂质体两性霉素B。脂质体两性霉素B制剂含有5mg/ml两性霉素B和6.5mg/ml类脂体(7ml DMPC和3 mlDMPG)于生理盐水中。由于类脂体的干扰使微生物法测定两性霉素B效价结果偏低,因此在测定前需将类脂体预先分离除去,常规的萃取方法分离类脂体的效果不佳,从TLC实验表明,硅胶板用氯仿:甲醇:丙酮:水:氨水(65∶30∶5∶2∶2)展开时二个类脂体和药物之间的迁移行为有较大差别,提示可用硅胶 Amphotericin B is an important antifungal antibiotic, in order to reduce its toxicity, the use of phospholipids (dimyristoylphosphatidylcholine DMPC and dimyristoylphosphatidylglycerol DMPG) are combined into liposomal amphotericin B. Liposomal amphotericin B formulation contained 5 mg / ml amphotericin B and 6.5 mg / ml lipid (7 ml DMPC and 3 ml DMPG) in physiological saline. Due to the interference of lipids, the titration of amphotericin B by the microbiological assay is low, so the liposomes should be pre-separated and removed before assaying. Conventional extraction methods have poor performance in separating lipid bodies. From TLC experiments , Silica gel plate with chloroform: methanol: acetone: water: ammonia (65:30:5:2:2) when the two lipids and drug migration behavior between the larger differences, suggesting that available silica gel