AIM:To express Hsp60 protein of H pylori by a constructedvector and to evaluate its immunogenicity.METHODS:Hsp60 DNA was amplified by PCR and insertedinto the prokaryotie expression vector pET-22b (+),whichwas transformed into BL21 (DE3) E.coli strain to expressrecombinant protein.Immunogenicity of expressed Hsp60protein was evaluated with animal experiments.RESULTS:DNA sequence analysis showed Hsp60 DNAwas the same as GenBank’s research.Hsp60 recombinantprotein accounted for 27.2 % of the total bacterial protein,and could be recognized by the serum from H pylori infectedpatients and Balb/c mice immunized with Hsp60 itself.CONCLUSION: Hsp60 recombinant protein might become a potential vaccine for controlling and treating Hpy/oriinfection. AIM: To express Hsp60 protein of H pylori by a constructed vector and to evaluate its immunogenicity. METHODS: Hsp60 DNA was amplified by PCR and inserted into the prokaryotic expression vector pET-22b (+) which transformed into BL21 (DE3) E. coli strain to expressrecombinant protein. Immunogenicity of expressed Hsp60 protein was as with animal experiments .RESULTS: DNA sequence analysis showed Hsp60 DNA was the same as GenBank’s research. Hsp60 recombinant protein accounted for 27.2% of the total bacterial protein, and could be recognized by the serum from H pylori infectedpatients and Balb / c mice immunized with Hsp60 itself.CONCLUSION: Hsp60 recombinant protein might become a potential vaccine for controlling and treating Hpy / oriinfection.