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目的 :探讨血清肝纤维化指标 型前胶原 ( PC )、IV型胶原 ( CIV)、透明质酸( HA)水平与大鼠肝脏组织学纤维化程度的关系。方法 :建立复合病因致大鼠肝纤维化的模型 ,分别于 2周、4周、6周、8周末随机取一组大鼠 ,用放射免疫法测定其血清 PC 、CIV、HA的水平 ,并观察了大鼠肝脏组织学变化规律及电镜下肝星状细胞数量、形态的改变。结果 :造模过程中 ,大鼠血清 PC 、CIV、HA水平均不断升高 ,从第 4周起较正常对照组显著升高。 2周、4周、6周、8周时大鼠肝脏组织学纤维化程度评分分别为 0 .63± 0 .52、1 .75± 0 .71、2 .63± 0 .92、3 .2 2± 0 .83。大鼠血清 PC 、CIV、HA与肝脏组织学改变的相关系数分别为 :0 .578,0 .677,0 .82 0 ,P均小于 0 .0 0 1。电镜下可见模型组大鼠肝星状细胞被激活 ,数量显著增多 ,形态亦有明显改变。结论 :血清 PC 、CIV、HA的水平可作为反映肝纤维化程度的指标。
Objective: To investigate the relationship between the level of serum hepatic fibrosis index procollagen (PC), type IV collagen (CIV) and hyaluronic acid (HA) and the degree of liver fibrosis in rats. Methods: A rat model of hepatic fibrosis induced by compound etiology was established. One group of rats were randomly selected at 2 weeks, 4 weeks, 6 weeks and 8 weeks respectively. Serum levels of PC, CIV and HA were measured by radioimmunoassay The histological changes of rat liver and the number and morphology of hepatic stellate cells under electron microscope were observed. Results: The levels of serum PC, CIV and HA in rats during the modeling were continuously increased, from the 4th week onwards, they were significantly higher than those of the normal control group. At 2 weeks, 4 weeks, 6 weeks and 8 weeks, the histological scores of liver fibrosis in rats were respectively 0.63 ± 0.52,1.75 ± 0.71,2.63 ± 0.92,3.2 2 ± 0 .83. The correlation coefficients of serum PC, CIV, HA and liver histological changes in rats were: 0.578,0.677,0.882, P were all less than 0.010. Electron microscope shows that the model group of rat hepatic stellate cells are activated, the number increased significantly, the morphology also changed significantly. Conclusion: The levels of serum PC, CIV and HA can be used as an index to reflect the degree of liver fibrosis.