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目的:研究乳腺癌术后发生甲状腺癌患者乳腺癌组织中RET(rearranged during transfection)基因的表达及临床意义。方法:用逆转录-聚合酶链反应(RT-PCR)检测33例乳腺、甲状腺多原发癌患者乳腺癌组织中RET基因的酪氨酸激酶区(TK)的表达进行分析,并设30例单纯乳腺癌和20例乳腺良性病变做对照组。结果:(1)乳腺、甲状腺多原发癌患者乳腺癌组织中RET-TK阳性检测率达57.6%(19/33),单纯乳腺癌患者癌组织中RET-TK阳性检出率30%(9/30),乳腺良性病变患者病变乳腺组织中RET-TK阳性检出率为10%(2/20)。三组的阳性率差别分别都有统计学意义(P<0.05)。(2)RET-TK的阳性表达与患者的ER情况相关(P<0.05),而与患者年龄、肿瘤直径、同侧腋窝淋巴结转移、Her-2表达等病理学特征无关(P>0.05)。结论:RET基因的过量表达可能与乳腺、甲状腺多原发癌的发生有关。
Objective: To study the expression and clinical significance of RET (rearranged during transfection) gene in breast cancer patients with thyroid carcinoma after operation. Methods: RT-PCR was used to detect the expression of tyrosine kinase domain (TK) of RET gene in 33 cases of breast and thyroid multiple primary breast cancers, and 30 cases Simple breast cancer and 20 cases of benign breast disease control group. Results: (1) The positive rate of RET-TK in breast cancer tissues with multiple primary thyroid cancer was 57.6% (19/33), and the positive rate of RET-TK in breast cancer patients with simple primary breast cancer was 30% (9 / 30). The positive rate of RET-TK in breast lesions of benign breast lesions was 10% (2/20). The positive rates of the three groups were statistically significant (P <0.05). (2) The positive expression of RET-TK was correlated with the ER status of patients (P <0.05), but not with the age, tumor diameter, ipsilateral axillary lymph node metastasis and Her-2 expression (P> 0.05). Conclusion: The overexpression of RET gene may be related to the occurrence of multiple primary cancers of the breast and thyroid.