[Objective] This study aimed to evaluate the healthy risk of genetically modified (GM) soybeans by using a novel approach for functions and safety of food.[Method] Different from traditional evaluation of substantial equivalence, three great innovations were performed in this study, involving in basic diet, evaluation approaches and principle, as well as the clarification of connotation differences between absolute and relative mass of organs. Hence a novel BDI-GS (Benefit- Damage Index and General Score) evaluation approach was established and applied in comparative evaluation between RR GM and natural soybeans. Healthy male ICR mice during linear growth were selected; experimental mice were fed with 15% RR GM soybeans and 15% natural soybeans blending maize meal diets, and control mice were fed with single maize meal diet for 13 d; the mice were dissected after collecting blood samples and perfectly obtained nine organs or tissues to record their masses and conduct statistical analyses.[Result] Plenty of matching information was obtained through simple design. The growth performance of treated mice was markedly of individual differences, some mice were thwarted due to regular intake of RR soybeans. Meanwhile, the functions and safety of RR soybeans were markedly lowered in overall nutritional and healthy effects than those of natural soybeans expressed in GS values, and presents some declines in nutrition and health of thymus, pancreas and spermary; especially, it can make thymus immune (P<0.05) in markedly lower level than that of natural soybeans.[Conclusion] Therefore, major troubles and risks of RR soybeans intake are of personal risks in different degrees, in addition, it may increase sub-health and related chronic epidemics risks, and herein it will presents certain safety issues. The creation of this novel evaluation system provides a simple and available evaluation approach for functions and potential risks revelation of food effects, and will yield far-reaching influences to safety evaluation and healthy development of GM foods, as well as public health. [Objective] This study aimed to evaluate the healthy risk of genetically modified (GM) soybeans by using a novel approach for functions and safety of food. [Method] Different from traditional evaluation of substantial equivalence, three great innovations were performed in this study, involving in basic diet, evaluation approaches and principle, as well as the clarification of connotation differences between absolute and relative mass of organs. Thus a novel BDI-GS (Benefit-Damage Index and General Score) evaluation approach was established and applied in comparative evaluation between RR GM and natural soybeans. Healthy male ICR mice during linear growth were selected; experimental mice were fed with 15% RR GM soybeans and 15% natural soybeans blending maize meal diets, and control mice were fed with single maize meal diet for 13 days ; the mice were dissected after collecting blood samples and perfectly obtained nine organs or tissues to record their masses and conduct statistical analysis es. [Result] Plenty of matching information was obtained through simple design. The growth performance of treated mice was markedly of individual differences, some mice were thwarted due to regular intake of RR soybeans. Meanwhile, the functions and safety of RR soybeans were markedly lower in overall nutritional and healthy effects than those of natural soybeans expressed in GS values, and presents some declines in nutrition and health of thymus, pancreas and spermary; especially, it can make thymus immune (P <0.05) in markedly lower level than that of natural soybeans. [Conclusion] Therefore, major troubles and risks of RR soybeans intake are of personal risks in different degrees, in addition, it may increase sub-health and related chronic epidemics risks, and it it will presents certain safety issues. creation of this novel evaluation system provides a simple and available evaluation approach for functions and potential risks revelation of food effects, and will yield far-reaching influences to safety evaluation and healthy development of GM foods, as well as public health.