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目的研究胰腺B细胞显像剂99Tcm-DTPA-NGN2在小鼠体内的分布规律,为99Tcm-DTPA-NGN2用于临床胰腺B细胞显像及胰腺癌的核医学显像做基础。方法正常小鼠42只,分为7组,每组6只,尾静脉注射99Tcm-DT-PA-NGN23.7MBq后5、15、30min和1、2、4、6h放血处死,取心、肝、脾、肺、脑、肾、肌肉、骨、小肠、胃、胰腺和血液等组织或器官,称重并测量其放射性,计算每克组织百分注射剂量率(%ID/g)。同时,取正常小鼠5只,尾静脉注射99Tcm-DTPA-NGN218.5MBq后相同时间进行显像。结果99Tcm-DTPA-NGN2主要经肾脏代谢,脑内未见摄取,各组织、器官的百分注射剂量率在1h内均迅速下降,胰腺的百分注射剂量率随时间的延长逐渐上升,在1～2h内保持较高水平。显像结果与生物分布实验结果一致,随时间的延长胰腺显影逐渐清晰,在1～2h内均可清晰观察胰腺组织。结论99Tcm-DTPA-NGN2可能是一种较好的胰腺B细胞显像剂,在胰腺B细胞的功能显像和胰腺癌显像的应用中值得进一步研究。
Objective To study the distribution of 99Tcm-DTPA-NGN2, a pancreatic B cell imaging agent, in mice for the imaging of 99Tcm-DTPA-NGN2 for clinical pancreatic B-cell imaging and nuclear imaging of pancreatic cancer. Methods Forty-two normal mice were randomly divided into 7 groups with 6 mice in each group. After caudal vein injection of 99.7cm-DT-PA-NGN at 23.7MBq, mice were sacrificed at 5, 15, 30min and 1, 2, (% ID / g) per gram of tissue were weighed and their radioactivity was measured by measuring the radioactivity of the tissue or organ in the spleen, lung, brain, kidney, muscle, bone, small intestine, stomach, pancreas and blood. At the same time, 5 normal mice were taken and irradiated with 99Tcm-DTPA-NGN218.5MBq through the tail vein for imaging at the same time. Results 99Tcm-DTPA-NGN2 was mainly metabolized by the kidneys and no intracerebral uptake was observed. The dose rate of each tissue and organ decreased rapidly within 1 h. The percent of injected dose of pancreas gradually increased with time, ~ 2h to maintain a high level. The imaging results were consistent with the results of the biodistribution experiment. The pancreas developed gradually with the passage of time, and the pancreas tissues were clearly observed within 1-2 h. Conclusion 99Tcm-DTPA-NGN2 may be a better imaging agent for pancreatic B cells. It is worth further study in functional imaging of pancreatic B cells and imaging of pancreatic cancer.