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目的:研究抑制核因子κB(NFκB)活性对实验性糖尿病大鼠肾组织一氧化氮(NO)水平的影响。方法:雄性Wistar大鼠分为3组,A组(11只)为正常对照组,B组(11只)为糖尿病未干预组,C组(9只)为糖尿病大鼠吡咯烷二硫基甲酸酯(PDTC,NFκB活性抑制剂)干预组。以链脲佐菌素(STZ)制备糖尿病大鼠模型。大鼠饲养18周后取出肾脏以电泳迁移率变动分析技术检测NFκB活性,RT PCR技术检测诱导型NO合成酶(iNOS)mRNA表达并检测肾组织NO水平,同时电镜检测大鼠肾小球基底膜厚度及系膜基质密度(系膜基质面积/系膜面积)。采用大鼠白蛋白特异的酶免疫分析试剂盒检测24h尿白蛋白排泄(UAE)。结果:肾组织iNOSmRNA表达在B组大鼠(0.30±0.12)显著高于A组(0.12±0.04,P<0.01)和C组(0.16±0.08,P<0.01)。肾组织NO水平在B组大鼠(0.56±0.20μmol/mg肾组织)显著低于A组(1.05±0.25μmol/mg肾组织,P<0.01)和C组(1.45±0.61μmol/mg肾组织,P<0.01)。基底膜厚度在B组大鼠(531.6±107.6nm)显著高于A组(312.4±25.4nm,P<0.05)和C组(315.8±21.4nm,P<0.05)。系膜基质密度在B组大鼠(56.41±6.78)显著高于A组(33.95±5.22,P<0.05)和C组(37.97±7.37,P<0.05)。结论:在实验糖尿病大鼠,肾组织iNOSmRNA表达明显增加,但NO水平明显降低;抑制NFκB活性不但能延?
Objective: To investigate the effect of inhibiting nuclear factor κB (NFκB) activity on the level of nitric oxide (NO) in the kidney of experimental diabetic rats. Methods: Male Wistar rats were divided into three groups: group A (n = 11) as normal control group, group B (n = 11) without diabetic intervention, group C Acid ester (PDTC, NFκB activity inhibitor) intervention group. Diabetic rats were induced by streptozotocin (STZ). After 18 weeks of rearing, the kidneys were removed and the activity of NFκB was detected by electrophoretic mobility shift assay. The expression of iNOS mRNA was detected by RT PCR and the level of NO in renal tissue was detected by RT-PCR. Meanwhile, the glomerular basement membrane Thickness and mesangial matrix density (mesangial matrix area / mesangial area). Urine albumin excretion (UAE) was detected by rat albumin-specific enzyme immunoassay kit. Results: The expression of iNOS mRNA in renal tissue was significantly higher in group B than in group A (0.12 ± 0.04, P <0.01) and in group C (0.16 ± 0.08, P <0.01). The level of NO in kidney in group B was significantly lower than that in group A (1.05 ± 0.25μmol / mg, P <0.01) and group C (1.45 ± 0.61μmol / mg) , P <0.01). Basement membrane thickness was significantly higher in group B (531.6 ± 107.6nm) than in group A (312.4 ± 25.4nm, P <0.05) and group C (315.8 ± 21.4nm, P <0.05). The density of mesangial matrix in group B (56.41 ± 6.78) was significantly higher than that in group A (33.95 ± 5.22, P <0.05) and group C (37.97 ± 7.37, P <0.05). Conclusion: In experimental diabetic rats, the expression of iNOS mRNA in kidney increased obviously, but the level of NO decreased obviously. Inhibition of NFκB activity not only prolonged,