目的 :探讨体外重建角膜组织的三维培养技术。方法 :原代培养角膜上皮、基质和内皮细胞 ,利用硫酸软骨素、透明质酸钠、 型胶原蛋白和微孔材料 ,建立三维培养系统 ,体外重建角膜组织结构 ,观察非醛交联、醛交联和微孔材料等三类人工角膜的透明度 ,常规病理组织学检测细胞在三维培养系统中生长状态。结果 :非醛交联类重建人工角膜质地较软 ,透明度较低 ,光镜可见纤维结构。醛交联类重建人工角膜质地较韧 ,透明性较高 ,光镜可见均质结构。非吸收微孔材料类细胞长入较少 ,较难体外重建人工角膜。结论 :利用生物工程技术能够体外重建人工角膜 ,非醛交联类结构较规则 ,醛交联类透明度较高 Objective: To investigate the three-dimensional culture technology of reconstructed corneal tissue in vitro. Methods: The corneal epithelium, stroma and endothelial cells were cultured in primary culture. Three-dimensional culture system was established by using chondroitin sulfate, sodium hyaluronate, collagen and microporous material. The corneal tissue was reconstructed in vitro. Combined with microporous materials, three kinds of corneal transparency, routine histopathology cells in three-dimensional culture system in the growth state. Results: Non-aldehyde cross-linked reconstructive artificial cornea texture is soft, low transparency, fiber structure can be seen by light microscopy. Aldehyde cross-linked reconstructive prosthetic corneal texture more tough, higher transparency, light microstructure can be seen homogeneous. Non-absorbable microporous material cells grow into less difficult to reconstruct the cornea in vitro. Conclusion: Bioengineering can reconstruct cornea in vitro. The non-formaldehyde cross-linked structure is more regular and the cross-linked aldehyde is more transparent