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应用自制的直径２０ｎｍ的胶体金标记ＧＡＲ－ＩｇＧ，以斑点免疫金银染色法（Ｄｏｔ－ＩＧＳＳ）、斑点免疫金染色法（Ｄｏｔ－ＩＧＳ）和Ｄｏｔ－ＥＬＩＳＡ三种方法，同时检测９９例脑囊虫病人脑脊液（ＣＳＦ）中循环抗原（ＣＡｇ），阳性率分别为９４．９５％、９３．９４％和９２．９３％。检测５１份非囊虫病患者和６２例其他脑部疾病患者的ＣＳＦ，三种方法均为阴性。诊断指数分别为１９４．９５％、１９３．９４％和１９２．９３％。检出ＣＡＳ的最小量Ｄｏｔ－ＩＧＳＳ为２４４ｎｇ／ｍｌ．Ｄｏｔ－ＩＧＳ和Ｄｏｔ－ＥＬＩＳＡ为４８８ｎｇ／ｍｌ。试验表明三种方法诊断脑囊虫病具有敏感性高、特异性强、重复性好和方法简便易行．有希望用于脑囊虫病的临床诊断。
The GALL-IgG with a diameter of 20 nm and a dot-immunogold staining (Dot-IGSS) and dot-immunogold staining (Dot-IGS) Cerebrospinal fluid (CSF) circulating antigen (CAg), the positive rates were 94.95%, 93.94% and 92.93%. CSF was detected in 51 non-cysticercosis patients and 62 other brain disease patients, all of which were negative. The diagnostic indexes were 194.95%, 193.94% and 192.93% respectively. The minimum amount of detected Dot-IGSS was 244 ng / ml. Dot-IGS and Dot-ELISA was 488 ng / ml. Tests show that the three methods of diagnosis of cerebral cysticercosis with high sensitivity, specificity, good reproducibility and method is simple and easy. Hopefully for the clinical diagnosis of cysticercosis.