前言对人类病毒致病原作鉴定,必需阐明它的基因结构和复制方式。要做到这点,首先要进行病毒的分子克隆和序列分析,然后再用已有的病毒特异性探针作出更详尽的研究。以前进行这类研究时,需要有大量的、足够滴度的病毒材料,才能确保病毒基因组分子克隆成功。通过体外细胞培养使病毒表达和增殖,也是一种替代活材料分离病毒特异性克隆的办 Preface Identification of human pathogens of the virus must be clarified about its genetic structure and replication. To do this, first make molecular cloning and sequence analysis of the virus, and then use the existing virus-specific probe to make more detailed studies. In the past, such studies required large amounts of viral material of sufficient titers to ensure successful cloning of viral genomes. By in vitro cell culture so that the virus expression and proliferation, but also an alternative to live virus isolation of virus-specific clones