以某再生水供给的模拟管段形成的生物膜为对象,在次氯酸钠投量为15 mg/L条件下,研究高浓度消毒剂对生物膜中微生物及其胞外聚合物含量的影响、不同类型微生物对消毒剂的敏感性及其活性恢复情况。结果表明,随着高浓度消毒剂作用时间的延长,生物膜微生物的存活率降低,但仍有部分微生物可以存活并得到恢复;消毒3 h及无氯恢复7 d后生物膜EPS总含量逐步降低;消毒剂对不同类型微生物的杀灭率也不同,其中亚硝酸盐氧化菌最易被杀灭;消毒3 h后,生物膜的硝化活性丧失且未见恢复,而反硝化活性虽大幅降低但可恢复。因此,采用高浓度消毒剂消毒并不能完全控制再生水管道生物膜微生物。 The biofilm formed from a simulated tube section of a given reclaimed water was used to study the effects of high concentrations of disinfectants on the content of microorganisms and extracellular polymers in the biofilm at a sodium hypochlorite dosage of 15 mg / Disinfectant sensitivity and recovery of activity. The results showed that with the prolongation of high concentration disinfectant, the survival rate of biofilm microorganisms decreased, but some microorganisms survived and recovered. The total EPS content of biofilms gradually decreased after 3 h of disinfection and 7 days of no chlorine treatment ; Disinfectants kill different types of microorganisms are also different, of which nitrite oxidation bacteria most likely to be killed; disinfection 3 h after the loss of nitrification activity and no recovery of the biofilm, while the denitrification activity was significantly reduced but Can be restored. Therefore, disinfection with high concentrations of disinfectant does not completely control the biofilm microorganisms in the reclaimed water pipeline.