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[目的]探讨牛磺酸对尼古丁所致雄性大鼠睾丸氧化损伤的保护作用。[方法]32只SD雄性大鼠,随机分为阴性对照组、牛磺酸对照组、尼古丁染毒组及牛磺酸干预组。阴性对照组大鼠生理盐水1 m L皮下注射,双蒸水1 m L灌胃;牛磺酸对照组大鼠皮下注射生理盐水1 m L,灌胃牛磺酸双蒸水溶液1 m L(牛磺酸量为100 mg/kg);尼古丁染毒组大鼠皮下注射尼古丁生理盐水溶液1 m L(尼古丁量为2 mg/kg),灌胃双蒸水1 m L;牛磺酸干预组大鼠如上注射尼古丁生理盐水溶液和灌胃牛磺酸双蒸水溶液。皮下注射和灌胃每日1次,连续4周。测定大鼠睾丸系数、附睾系数、精子密度和活率;检测睾丸组织丙二醛(MDA)含量;检测睾丸组织超氧化物歧化酶(SOD)、过氧化氢酶(CAT)和谷胱甘肽过氧化物酶(GSH-Px)活性。[结果]睾丸系数、附睾系数、精子密度和活率:尼古丁染毒组大鼠显著低于另3组(P<0.05),牛磺酸干预组显著低于阴性对照组和牛磺酸对照组(P<0.05);睾丸组织MDA含量:尼古丁染毒组大鼠显著高于另3组(P<0.05),牛磺酸干预组显著高于阴性对照组和牛磺酸对照组(P<0.05);睾丸组织SOD、CAT、GSH-Px活性:尼古丁染毒组大鼠显著低于另3组(P<0.05),牛磺酸干预组显著低于阴性对照组和牛磺酸对照组(P<0.05)。[结论]尼古丁可致大鼠睾丸组织氧化损伤,牛磺酸对此具有抗氧化保护作用。
[Objective] To explore the protective effect of taurine on oxidative damage of testis in male rats induced by nicotine. [Methods] Thirty-two SD male rats were randomly divided into negative control group, taurine control group, nicotine exposure group and taurine intervention group. The rats in the negative control group were subcutaneously injected with 1 m L of physiological saline and 1 m L of distilled water. The taurine control rats were injected subcutaneously with normal saline (1 m L) The amount of sulfonic acid was 100 mg / kg). The nicotine-exposed rats were subcutaneously injected with 1 mL of nicotine saline solution (2 mg / kg of nicotine) Rats were injected with nicotine saline and gavage with taurine double distilled water. Subcutaneous injection and gavage once daily for 4 weeks. The testis coefficient, epididymis coefficient, spermatozoa density and viability were determined. The contents of malondialdehyde (MDA) in testis tissue were measured. The contents of superoxide dismutase (SOD), catalase (CAT) and glutathione Peroxidase (GSH-Px) activity. [Results] Testis coefficient, epididymal coefficient, spermatozoa density and viability were significantly lower in nicotine-treated rats than those in the other three groups (P <0.05), taurine-treated group was significantly lower than that in the negative control group and taurine control group (P <0.05). The content of MDA in testis tissue was significantly higher in the nicotine group than that in the other three groups (P <0.05). The taurine intervention group was significantly higher than the negative control group and the taurine control group (P <0.05). The activities of SOD, CAT and GSH-Px in testis tissue were significantly lower in nicotine-treated rats than those in the other three groups (P <0.05), and taurine-treated rats were significantly lower than those in the negative control group and the taurine control group (P <0.05) . [Conclusion] Nicotine can cause the oxidative damage of testicular tissue in rats, and taurine has anti-oxidant protective effect.