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为了研究运动训练大鼠不同部位组织(重点是脂肪组织)和器官的自体荧光光谱特性。方法:通过激光诱导荧光光谱技术测量一次中等强度力竭运动后大鼠组织的自体荧光光谱,并在肠系膜部位的脂肪组织的三维荧光光谱中发现了和运动相关的特有荧光峰,并判别出此荧光峰对应的荧光物质是NADH(还原态烟酰胺腺嘌呤二核苷酸),且研究了运动状态与峰强的相关性。结果:中等强度运动后大鼠肠系膜部位的脂肪组织的能量代谢强于大网膜部位的脂肪组织、皮下脂肪组织,并且强于前腿肌肉、比目鱼肌及其它脏器;NADH自体荧光光谱特性是判断机体组织代谢程度的有效的指标之一。
In order to study the autofluorescence spectral characteristics of different tissues (mainly adipose tissue) and organs in different regions of exercise training rats. METHODS: Autofluorescence spectra of rat tissues after a moderate-intensity exhaustive exercise were measured by laser-induced fluorescence spectroscopy, and the unique fluorescence peak associated with movement was found in the three-dimensional fluorescence spectrum of the adipose tissue in the mesentery. The fluorescent material corresponding to the fluorescence peak is NADH (reduced nicotinamide adenine dinucleotide), and the correlation between exercise state and peak intensity was studied. Results: The energy metabolism of adipose tissue in mesentery of rats after middle-intensity exercise was stronger than that in omentum and subcutaneous adipose tissue of the omentum and stronger than that of the front leg muscle, soleus muscle and other organs. Autofluorescence characteristics of NADH One of the effective indicators to judge the degree of body tissue metabolism.