人羊膜上皮细胞ＦＬ系分别接触ａ－萘黄酮（０．６ｍｍｏｌ·Ｌ￣（－１））β－萘黄酮（２０ｐｍｏｌ·Ｌ￣（－１））２４ｈ后，再用苯并（ａ）芘［Ｂ（ａ）Ｐ，１０ｕｍｏｌ·Ｌ￣（－１）］处理２４ｈ，用３２Ｐ后标记技术测定以Ｂ（ａ）－ＤＮＡ加合物。结果发现，阳性对照组，ａ－萘黄酮预处理组及β－萘黄酮预处理组加合物的量分别为（加合物个数／１０’个核苷酸）：４．７±０．２（１００％），１．８±０．９（３８．３％），１６．０±２．２（３４０．１％）．该实验结果直接显示了纳胞色素Ｐ４５０调节剂对肿瘤发生影响的作用水平。亦为药物对致癌物代谢影响的研究提供了一种方法． FL in human amniotic epithelial cells was exposed to β-naphthoflavone (20 pmol·L -1) for 24 hours and then treated with benzo [a] pyrene [ B (a) P, 10umol·L ~ (-1)] for 24 hours, and the B (a) -DNA adduct was determined by 32P labeling technique. The results showed that the amount of adduct in the positive control group, a-naphthoflavone pretreatment group and β-naphthoflavone pretreatment group were respectively (the number of adducts / 10 ’nucleotides): 4.7 ± 0. 2 (100%), 1.8 ± 0.9 (38.3%), 16.0 ± 2.2 (340.1%). The results of this experiment directly show the effect of cytochrome P450 modulators on tumorigenesis. It also provides a method for the study of the effects of drugs on carcinogen metabolism.