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目的：观察脑缺血再灌流过程中缺血灶和下丘脑的谷氨酸受体的变化特征。方法：应用高效液相色谱仪，受体的放射配体结合分析分别监测大脑中动脉阻塞再灌流大鼠缺血灶和下丘脑的谷氨酸（Ｇｌｕ）含量，ＧｌｕＲ容量及离解常数Ｋｄ变化。结果：缺血及再灌流全过程中，缺血灶的ＧｌｕＲ及其亲合力持续降低，而下丘脑呈现两次大幅度起伏变化，两部位Ｇｌｕ含量与ＧｌｕＲ容量均呈显著负相关（ｒ分别为－０．２３３和－０．３８５，Ｐ分别＜０．０５和０．００１）。结论：缺血早期缺血灶的ＧｌｕＲ有敏感的生物学活性，早期阻滞其ＧｌｕＲ对缺血性脑损伤有重要干预意义；缺血及再灌流全过程中，下丘脑的ＧｌｕＲ始终具有敏感的生物学活性，干预其ＧｌｕＲ对减轻缺血性应激性脑损伤有重要价值。
Objective: To observe the changes of glutamate receptors in ischemic and hypothalamus during cerebral ischemia and reperfusion. Methods: The contents of glutamic acid (Glu), the capacity of GluR and the Kd of dissociation constant in ischemic and hypothalamus of middle cerebral artery occlusion and reperfusion rats were detected by radioligand binding analysis of high performance liquid chromatography and radioimmunoassay. Results: In the whole process of ischemia and reperfusion, the GluR and its affinity in the ischemic lesion continued to decrease, while the hypothalamus showed two drastic ups and downs. There was a significant negative correlation between Glu content and GluR capacity in both sites (r -0.233 and -0.385 respectively, P <0.05 and 0.001, respectively). Conclusion: GluR in early ischemic ischemic lesion has sensitive biological activity, early block GluR on ischemic brain injury has an important intervention significance; during the whole process of ischemia and reperfusion, the GluR of the hypothalamus is always sensitive Biological activity, intervention GluR on the reduction of ischemic stress brain injury has important value.