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目的 :本文研究大鼠慢性低氧后心肌钙转运的变化。方法 :将大鼠放置于 10 %氧环境下 4周 ,分离正常及慢性缺氧大鼠的右心室肌细胞 ,用光谱荧光法测定电刺激和咖啡因引起的细胞内 [Ca2 + ]i瞬变 ,并测定肌浆网膜上的Ca2 + ATP酶 (SERCA)和ryanodine受体 (RyR)蛋白的水平。结果 :低氧后电刺激和咖啡因引起的细胞内 [Ca2 + ]i瞬变的幅度降低且时程延长 ,RyR水平无明显改变但SERCA已显著减少。结论 :Ca2 + ATP酶活性和蛋白水平的降低是低氧钙转运异常的主要原因。
Objective: To study the changes of calcium transport in rats after chronic hypoxia. METHODS: Right ventricular myocytes were isolated from normal and chronic hypoxia rats after being placed in 10% oxygen environment for 4 weeks. The changes of intracellular [Ca2 +] i induced by caffeine and electrical stimulation were measured by spectrofluorometry , And the level of sarcoplasmic reticulum Ca2 + ATPase (SERCA) and ryanodine receptor (RyR) protein were measured. Results: The amplitude of intracellular [Ca2 +] i transient induced by hypoxia and caffeine was decreased and the time course was prolonged. However, the level of RyR was not significantly changed but SERCA was significantly decreased. Conclusion: The decrease of Ca2 + -ATPase activity and protein level is the main reason of abnormal hypoxia-Ca2 + transport.